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| Rovani,Monique Tomazele; Cezar,Alfredo Skrebsky; Rigo,Melânia Lazzari; Gasperin,Bernardo Garziera; Nóbrega Júnior,Janduí Escarião da; Torres,Fabrício Dias; Gonçalves,Paulo Bayard Dias; Ferreira,Rogério. |
| ABSTRACT: Pregnancy diagnosis is an important tool for farm management. Ultrasonography is the main technique used for pregnancy diagnosis in ewes. As an alternative, radioimmunoassay (RIA) allows accurate and early detection of pregnancy-associated glycoproteins (PAGs) in sheep blood. However, radioactive-based techniques, as RIA, have been increasingly inadvisable due to environmental risk. Homology between ovine and bovine PAGs is high, and ELISA kits used for PAGs detection in cattle are safer than RIA. Thus, this study aimed to evaluate the feasibility of PAGs detection for pregnancy diagnosis in sheep serum samples using an ELISA kit produced for cattle. The sensitivity and specificity of the ELISA kit were 93.5% and 98.9%, respectively, whereas... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pregnancy-associated glycoproteins; Ewe; Pregnancy blood test; ELISA kit. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000200362 |
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| Rigo,Melânia Lazzari; Dau,Andressa Minussi Pereira; Glanzner,Werner Giehl; Martins,Manoel; Zanella,Renato; Rizzetti,Tiele Medianeira; Comim,Fabio Vasconcellos; Gonçalves,Paulo Bayard Dias. |
| The main objective of this study was to detect the steroidogenic effects of Ang II in bovine theca cells in vitro. Bovine theca cells were obtained from follicles (larger than 10mm of diameter) collected from a local abattoir and submitted to different treatments in a sequence of experiments. In experiment 1, CYP17A1 mRNA profile was evaluated in LH- (10ng ml-1) and Ang II-treated (0.1µM) theca cells. In experiment 2, a dose-response effect of Ang II (0.001; 0.1 e 10µM) plus insulin (100ng ml-1) and LH (100ng ml-1) was evaluated on steroidogenesis of bovine theca cells. Experiment 3 explored the effects of saralasin (an antagonist of Ang II receptors) on steroid production and steroidogenic enzymes regulation in theca cells. After 24 hours, culture media... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: RAS; LH; Steroidogenesis; Ovary. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000400704 |
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